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KMID : 1094720140190061042
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 6 p.1042 ~ p.1047
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Immobilization of acetyl xylan esterase on modified graphite oxide and utilization to peracetic acid production
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Yoo Hah-Young
Lee Ja-Hyun
Suh Young-Joon
Kim Sung-Bong
Park Seung-Moon
Kim Seung-Wook
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Abstract
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In the previous work, acetyl xylan esterase (AXE) of Aspergillus ficcum was successfully produced in Pichia pastoris as host. In this study, the recombinant AXE was immobilized on graphite oxide and used for the production of peracetic acid. Immobilization efficiency was enhanced by modifying graphite oxide via surface functionalization. The conditions for enzyme immobilization were also investigated and the optimal conditions were determined as 4℃ of temperature, 24 h of reaction time and pH 7. The activity of immobilized AXE was found to be 62.53 U/g-support. With the immobilized AXE, about 134 mM of peracetic acid was produced under 37℃ of temperature and 30 min of reaction time. Enzyme activity remained at > 50% of the original after 10 production cycles.
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KEYWORD
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acetyl xylan esterase, peracetic acid, enzyme immobilization, graphite oxide
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